Horse Cortisol ELISA Kit

Horse Cortisol ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to Cortisol, During the reaction,Cortisol in the sample or standard competes with a fixed amount of biotin-labeled Cortisol for sites on a pre-coated Monoclonal antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cortisol in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species: Horse ,Uniprot ID: , Storage: Store the whole kit at 4℃ , Intended Use: Horse Cortisol ELISA Kitallows for the in vitro quantitative determination of Cortisol , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids. , Alternative name: Cortisol ELISA Kit,Cortisol ELISA Kit,hydrocortisone ELISA Kit