Human ADAL ELISA Kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADAL. During the reaction,Human ADAL in the sample or standard competes with a fixed amount of biotin-labeled Human ADAL for sites on a pre-coated Monoclonal antibody specific to Human ADAL. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human ADAL in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species: Human Storage : Store the whole ELISA kit at 4℃