Human ASCC3 ELISA Kit

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species: Human Storage : Store the whole ELISA kit at 4℃