Human ACVB ELISA Kit

Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin B (ACVB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin B (ACVB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin B (ACVB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin B (ACVB) in the samples is then determined by comparing the O.D. of the samples to the standard curve. Species: Human Storage : For 5-7days:Store the whole kit at 4℃For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.